EGFP expression in the forebrain (epiphysis), spinal cord (anterior roof plate, dorsal neurons) and pectoral fins.

Genomic location: Chromosome 14

The insertion is 21.8 kb downstream of zic3

DNA sequence flanking Tol2 insertion site

3:CACGTAATTTGTTTCTTCAAGCTTTGTTTCTTAGCTTATCTAAGTTCTAG

TTGCAAGGTTGTACTCCATTGATTTATTTAACTTCAACTGTTTATAACAACT

TTTTTATTAAGTTAAAGGTTTGACACTGATTTGAACTTGAAGTGGCGATGAG

ATCTTAAAAACTTCTGCGAAGGTCTTTAAAAAGTCTTAAAAGGTATTGAAAT

TACTTGGATTCCAGA